diff --git a/libunwind/test/configs/llvm-libunwind-merged.cfg.in b/libunwind/test/configs/llvm-libunwind-merged.cfg.in
index 19808a173ad8..76b572c4cdbf 100644
--- a/libunwind/test/configs/llvm-libunwind-merged.cfg.in
+++ b/libunwind/test/configs/llvm-libunwind-merged.cfg.in
@@ -25,7 +25,6 @@ if @LIBUNWIND_USES_ARM_EHABI@:
     config.available_features.add('libunwind-arm-ehabi')
 
 if not @LIBUNWIND_ENABLE_THREADS@:
-    compile_flags.append('-D_LIBUNWIND_HAS_NO_THREADS')
     config.available_features.add('libunwind-no-threads')
 
 if @LIBUNWIND_ENABLE_CET@:
diff --git a/libunwind/test/configs/llvm-libunwind-shared.cfg.in b/libunwind/test/configs/llvm-libunwind-shared.cfg.in
index c0a3e4d922be..11c9faedf0b8 100644
--- a/libunwind/test/configs/llvm-libunwind-shared.cfg.in
+++ b/libunwind/test/configs/llvm-libunwind-shared.cfg.in
@@ -28,7 +28,6 @@ if @LIBUNWIND_USES_ARM_EHABI@:
     config.available_features.add('libunwind-arm-ehabi')
 
 if not @LIBUNWIND_ENABLE_THREADS@:
-    compile_flags.append('-D_LIBUNWIND_HAS_NO_THREADS')
     config.available_features.add('libunwind-no-threads')
 
 if @LIBUNWIND_ENABLE_CET@:
diff --git a/libunwind/test/configs/llvm-libunwind-static.cfg.in b/libunwind/test/configs/llvm-libunwind-static.cfg.in
index 0bb420730717..ce5a6c82caa0 100644
--- a/libunwind/test/configs/llvm-libunwind-static.cfg.in
+++ b/libunwind/test/configs/llvm-libunwind-static.cfg.in
@@ -29,7 +29,6 @@ if @LIBUNWIND_USES_ARM_EHABI@:
     config.available_features.add('libunwind-arm-ehabi')
 
 if not @LIBUNWIND_ENABLE_THREADS@:
-    compile_flags.append('-D_LIBUNWIND_HAS_NO_THREADS')
     config.available_features.add('libunwind-no-threads')
 else:
     link_flags.append('-lpthread')