25 lines
1.0 KiB
Plaintext
25 lines
1.0 KiB
Plaintext
Primer3 is a widely used program for designing PCR primers (PCR =
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"Polymerase Chain Reaction"). PCR is an essential and ubiquitous tool in
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genetics and molecular biology. Primer3 can design hybridization probes
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and sequencing primers.
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PCR is used for many different goals. Consequently, primer3 has many
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different input parameters that you control and that tell primer3
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exactly what characteristics make good primers for your goals.
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Primer3 picks primers for PCR reactions, considering as criteria:
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* oligonucleotide melting temperature, size, GC content and primer-dimer
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possibilities,
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* PCR product size
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* positional constraints within the source (template) sequence
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* possibilities for ectopic priming (amplifying the wrong sequence)
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* many other constraints.
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All of these criteria are user-specifiable as constraints, and some are
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specifiable as terms in an objective function that characterizes an
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optimal primer pair.
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manual online: https://primer3.org/manual.html
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For details and citation see "References" in the package's doc folder.
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